Table 1.
Primer and probe sequences used for quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR)
| Gene symbol | NM_Number | Probe name | Probe sequence | Forward name | Forward sequence | Reverse name | Reverse sequence |
|---|---|---|---|---|---|---|---|
| CD3 | NM_198053 | MP317 | CAAAGCTCTGTGCCTCTGTAATCGGCA | MP317_For | CACCGCGGCCATCCT | MP317_Rev | AGTTTGGGATCCAGCAGGC |
| CD8 | NM_001145873 | MP607 | TTCCTGCCAGCGAAGCCCAC | MP607_For | TGAGCAACTCCATCATGTACTTCAG | MP607_Rev | GGCGCCGGTGTTGGT |
| FOXP3 | NM_014009 | MP551 | TTTTCTGTCAGTCCACTTCACCAAGCCTG | MP551_For | CCCACAAGCCAGGCTGAT | MP551_Rev | GCATCGGGTCCTTGTCCA |
| CALM2 | NM_001743 | MP501 | TCGCGTCTCGGAAACCGGTAGC | MP501_For | GAGCGAGCTGAGTGGTTGTG | MP501_Rev | AGTCAGTTGGTCAGCCATGCT |