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. 2018 Sep 12;7(10):5155–5166. doi: 10.1002/cam4.1765

Figure 2.

Figure 2

Verification of LEA as PODXL. A, Immunological cross‐reactivity between LEA and PODXL in CL187 cells was analyzed by IP assay with ND‐1 (left) and 3D3 (right), respectively, and then cross‐detected by western blotting with 3D3 (left) and ND‐1 (right). LEA expression depended on PODXL gene was validated using an overexpression assay in HEK293 cells (B), and siRNA assay in CL187 cells (C). IB, immunoblotting; WB, western blotting; MW, molecular weight