Figure 4.

Tanshinone reduced tumor size and weight in mice models. A, Mice from irradiation and Tanshinone IIA co‐treatment group were sacrificed after 150 d. The tumors from animals in the other three groups were collected from the nude mice after they died. B, The growth curves of AMC‐HN‐8 generated tumors receiving different treatments including PBS (by tail vein injection), irradiation alone (20 Gy in three fractions every 4 d), Tanshinone IIA alone (by tail vein injection, 20 μmol/L daily for 12 consecutive days), or irradiation combined with Tanshinone IIA (irradiation was performed at day 4, 8, and 12 after injection of 20 μmol/L Tanshinone IIA which began at day 1 for 12 consecutive days). Tumor volume was calculated according to their diameters. *** indicated P < 0.001 by two‐way ANOVA. C, The weight of AMC‐HN‐8 generated tumors receiving different treatments as indicated. The weight of tumors was measured 10 days after the end of treatment. *** indicated P < 0.001 by two‐tailed unpaired Student's t test compared to the PBS group