Table 3.
Reproducibility Experiment: Sequencing Validation Design
| Assay | Day | Intra |
|||||
|---|---|---|---|---|---|---|---|
| Sequencer 1 | Sequencer 2 | Sequencer 3 | |||||
| Inter | Day 1 | CA-0101-p1 (frozen) CA-0122-p1 (frozen) |
ONC16-13-p1 (FFPE) GBM-35-p1 (FFPE) |
||||
| Day 2 | CA-0101-p1 (frozen) CA-0122-p1 (frozen) |
ONC16-13-p1 (FFPE) GBM-35-p1 (FFPE) |
CA-0101-p2 CA-0122-p2 |
ONC16-03-p2 GBM-35-p2 |
CA-101-p3 CA-0122-p3 |
ONC16-13-p3 GBM-35-p3 |
|
| Day 3 | CA-0101-p1 (frozen) CA-0122p1 (frozen) |
ONC16-13-p1 (FFPE) GBM-35-p1 (FFPE) |
|||||
Sequencing was performed on the same library preparation (p1) for four samples (two frozen and two FFPE) on the same sequencing machine on three separate days (inter-run reproducibility). Two additional library preparations were performed for each sample (p2, p3) and sequenced on the same day on different sequencing machines (intra-technician reproducibility).
FFPE, formalin-fixed, paraffin-embedded.