Table 4.

Minimum Number of Total Reads Required to Detect a Variant

Type	VAF	Total reads	Method
SNVs	15%	≥40	WGS
Indels	20%	≥40	WGS
CNVs	NA	≥30∗	WGS
SVs	NA	≥3	WGS
Fusions	NA	≥5†	RNA-seq

CNV, copy number variant; Indel, insertion/deletion; NA, not applicable; SNV, single-nucleotide variant; SV, structural variant; VAF, variant allele frequency; WGS, whole-genome sequencing.

^∗Based on Xi et al.²⁸

^†Spanning pairs.