Skip to main content
. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Leuk Lymphoma. 2016 Dec 8;58(7):1702–1710. doi: 10.1080/10428194.2016.1260122

Figure 4.

Figure 4.

Canine DLBCL TMA. Canine lymphoma TMA slides were stained for p52, p65, and RelB and scanned for the analysis. Manual annotations were drawn to segment the desired TMA spots into individual regions per the TMA maps. Negative annotations were drawn to exclude regions containing poor tissue, staining artifacts, and non-representative tissue (e.g. benign areas). p50, p65, RelB, and p52 antigens were present in tumor areas encompassing nuclear and cytoplasmic staining. The Nuclear aμgorithm was calibrated to the DAB/Hematoxylin stains and nuclear DAB staining was measured in tumor areas. (A) Representative photos with low and high frequency of NFκB nuclear translocation are shown. Blue dots represent unstained nuclei and yellow-orange dots represent positive NFκB staining in nuclei. (B) Summary of the percentage of NFkB positive nuclei in total nuclei among samples. Red color represents the result of normal lymph node samples.