Skip to main content
. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Adv Biol Regul. 2017 Mar 21;64:9–19. doi: 10.1016/j.jbior.2017.03.001

Fig. 6. A putative Mcm1-binding site is a transcriptional activator.

Fig. 6.

A) The sequence of the MFα2 promoter containing a putative Mcm1-binding site. The coordinates are indicated relative to the start codon of MFα2. The box encloses the putative Mcm1-binding site. The overlined nucleotides were mutated to adenosines to generate the Mcm1-binding site mutant (Mcm1 Site Mut.). B) Schematic of the construct used for studies of transcriptional activation. The grey box indicates the location of the MFα2 sequence that was cloned into pCM64 upstream of the minimal Cyc1 promoter and bacterial β-galactosidase (LacZ) gene. C) Assays of transcriptional activation in WT and IP pathway mutant strains. LacZ activity was measured in Miller Units as previously described (Ausubel, 1987). Data are presented as the fold induction over the values obtained from the empty vector in each strain. Values are the mean ± SEM of at least 6 independent cultures.