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. 2018 Oct 22;18:169. doi: 10.1186/s12935-018-0666-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — TALEN-mediated editing resulted in the reduction of E7 protein in both SiHa and CaSki cells. Immunocytochemical analysis revealed that levels of E7 protein was reduced in TALEN-treated (C, D) when compared to control (A, B) SiHa cells. Similarly, a reduction in E7 levels was observed in CaSki cells also after TALEN treatment (G, H) than in its respective control cells (E, F). This was further confirmed by Western blot analysis which showed a significant reduction in the levels of E7 after TALEN treatment in both the cell lines (I). Magnification-×600

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