Fig. 3.

Impact of G9a suppression on in vitro proliferation of NSCLC cells. a Western blot analysis (left panel) of cell lysates of A549, H1299, and H1975 cells transfected with either G9a siRNA or control siRNA and harvested at 72 h post transfection. Decrease in cell proliferation (right panel) upon knockdown of G9a (at 72 h post-transfection, P < 0.05 compared to control siRNA transfected). Restoration of HP1α expression in b A549 (upper panel), c H1299 (upper panel) partially abolished inhibitory effects of G9a knockdown on cell proliferation (down panel, P < 0.05 compared to sham-transfected cells). Effect of G9a inhibitor UNC0638 on H3K9Me2 (Upper panel for Western blot analysis, H3 for total H3 protein) the proliferation curve (lower panel) of d A549, e H1299, and f H1975 treated with defined UNC0638 for 72 h. *P < 0.05, ** P < 0.01 compared to control cells