Fig. 5.

Impact of UNC0638 treatment on Wnt activation and downstream molecules. a qRT–PCR for selected genes in A549 (left panel), H1299 (middle panel) and H1975 (right panel). Data is presented as the ratio to control sample (*P < 0.05, **P < 0.01, compared to control siRNA). b Western blot analysis of G9a, H3K9-Me2, APC2, DKK1, WIF1, and beta Catenin in the three cells treated with UNC0638 for 72 h. c TOPFlash-Luc reporter assays showed that knockdown of APC2 partially attenuated the inhibitory effect of UNC0638 on Wnt3a-induced reporter activity in both A549 cells (left panel) and H1299 cells (right panel). Bisulfite-PCR sequencing chromatogram for the representative CpG dimers and the methylation status (the black dots represent for methylated, the white for unmethylated CpG dimers) of the 30 CpG dimers in the APC2 promoter region (− 2840~ − 2560) of d A549, e H1299, and f H1975 cells. The upper panel of d-f is for cancer cells treated with vehicle, the lower panel is for cancer cells treated with UNC0638