Fig. 6.

Treatment of H1299 xenografts with UNC0638 strongly suppressed tumor growth and Wnt signaling pathway. a Tumor growth curves show UNC0638 treatment significantly inhibited H1299 xenografts growth. Xenograft growth was measured in vivo during 28 days, and treatment started at 10 days post-transplantation. b Resected tumors (three replicates) from different treatment regimens were illustrated for growth response to UNC0638 treatment (upper for vehicle, lower left and right for 5 and 10 mg/ml UNC0638). c Tumor weight in the mice treated with control (vehicle) and UNC0638 (*P < 0.05, compared to controls). d IHC stains for H3K9-Me2, APC2, and WIF1 in these xenograft tissues. IHC analysis shows that treatment with UNC0638 strongly increased APC2 and WIF1 proteins, concurrently decreased H3K9-me2 and β–catenin (magnification, × 200; red bar, 50 μm)