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. 2018 Aug;7(2):138–147. doi: 10.2174/2211536607666180416152112

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© 2018 Bentham Science Publishers

This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (4) — Repeated Freeze/Thaw (F/T) negatively impacts miRNA detection in unprocessed samples but improves it after Heat/Freeze (H/F) cycle. (A) Comparison of array results from two independent unprocessed samples and one F/T unprocessed samples. A 3 ml serum aliquot was defrosted on ice and frozen at -80oC a total of 5 times, followed by 3 H/F cycles and miRNA array. Venn diagram of the three samples is shown in the top panel and regression analysis of one unprocessed sample and one F/T samples is shown in the bottom panel (based on Ct values). (B) Comparison of array results from H/F samples before and after F/T. Venn diagram of all three samples from the same subject is shown in the top panel. Regression analysis between one H/F sample and one H/F plus 5 F/T cycles is shown in the bottom panel (based on Ct values).