Table 1.
List of mutations associated with colistin resistance in this study. Mutations were detected by pairwise SNP comparisons between one pair of colistin-susceptible and -resistant strains from each patient and sequence alignment of pmrAB genes
| Patient | ST (Oxford) | Core SNP differencesa | pmr mutationsb | Studies reporting identical pmrAB mutations (first author and reference number) | SNPs outside pmr |
|---|---|---|---|---|---|
| 1 | 208/208 | 6 | lptF, cyaA, yjaC, topA and deaD | ||
| 2 | 208/208 | 3 | L20F (pmrA) | cyaA, adeI | |
| 3 | 281/208 | 5720 | |||
| 4 | 208/208 | 17 | M12I (pmrA) | Arroyo28 | pgaC |
| 5 | 208/208 | 7 | R134S, T235I | Arroyo28 | cyaA, mmgC, mexA and itrA2 |
| 6 | 281/281 | 40 | A226T | pilY and zinc binding alcohol dehydrogenase | |
| 7 | 208/208 | 7 | – | znuA, yaiI, smvA, cyaA, ubiH, yecA and edd | |
| 8 | 281/208 | 7273 | F105L (pmrA) | ||
| 9 | 208/208 | 4 | T232A, F267L, L271F | gtr5, mfs | |
| 10 | 208/208 | 2 | Q277K | ||
| 11 | 208/208 | 5 | znuA, lptC, mfs and nlhH | ||
| 12 | 208/208 | 84 | P119L, R134S, T235I | Arroyo28 | |
| 13 | 208/208 | 3 | L208R, R263C | Arroyo28 | itrA2 |
| 14 | 281/208 | 7642 | pilY | ||
| 15 | 208/208 | 3 | P233T, L292H | Adams29 | |
| 16 | 208/208 | 7 | clpA, tolR, sel1 and mfs | ||
| 17 | 208/208 | 313 | A14V (pmrA) | ||
| 18 | 451/451 | 1 | P233S | Adams29; Beceiro30; Arroyo28; Kim31 | gna |
| 19 | 451/451 | 16 | G315V | tetR, wzc, gna, asnC and pgap1 | |
| 20 | 1557/208 | 44 830 | V444A | ||
| 21 | 281/348 | 7488 | – |
a
Core SNP counts include only genome sequences shared by all 42 isolates. Upstream part of pmrB was truncated in reference genome S1 and was excluded from core SNP analysis.
b
Mutations refer to pmrB except when pmrA is indicated inside parenthesis.