Matrix deposition in fused tissue tubes. Longitudinal sections of tubes cultured in static conditions for 14 days (A, B), or in static conditions for 7 days followed by 7 days of dynamic culture with ∼12 dyne/cm2 of applied shear (C, D). Picrosirius red fast green stain shown in (A, C; red = collagen, green = counterstain), orcein stain shown in (B, D; dark pink = elastic fibers, purple = nuclei). Lumen on bottom of image. Scale = 100 μm. Color images available online at www.liebertpub.com/tea