FIG. 6.

Differential COLII expression. Immunohistochemistry staining of scaffold pairs cultured in 5 ng/mL TGFβ3. (A) NT and scrambled shRNA; (B) NT and SMAD3 shRNA; (C) NT and scrambled shRNA with RUNX2; (D) NT and SMAD3 shRNA with RUNX2. (E) Human osteochondral control. (F) Representative negative control with no primary antibody. Square brackets indicate scaffold pairs cultured in the same well. Type II collagen-rich matrix was observed in both NT and scrambled shRNA-transduced scaffolds, while differential type II collagen staining was observed for all the other groups. Scale bar = 100 μm. Color images available online at www.liebertpub.com/tea