Fig. 1B.

Distribution and kinetics of NO metabolites in the tissues (heart, lungs liver and kidney). Distribution and kinetics of accumulation of NO metabolites in mice breathing NO (heart, lungs, liver and kidney). Concentrations of NO metabolites were measured in tissues of mice breathing air with 80 ppm NO for 60 min, harvested at 0, 10, 30 min, 1, 3, 6, 12, 24 and 48 h (NO, n = 3–7). Control mice breathed air for 60 min (n = 4–5). *P < 0.05 versus mice not breathing NO. C = control group. NO-heme = nitrosyl-heme species; RNNO = N-nitrosamines; RSNO = S-nitrosothiols. Additional information regarding each concentration, the number of animals studied, and the P value are available in the Supplemental Tables 2-4.