Figure 3. CCL5-induced signaling is not blocked by chemokine receptor antagonists.
RA-treated SH-SY5Y cells were exposed to DAPTA (20 nM), BX471 (25 nM), SB328437 (80 nM) 15 min prior to CCL5 (100 nM). Cells were lysed and induction of pAKT was determined by western blot analysis (A) as described in Materials and Methods. B. Data are the mean + SD of four samples (two separate samples for each compound, from two independent experiments). **p<0.01, ***p<0.001 vs control or respective antagonists.