Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Neurochem. 2018 Aug 2;146(5):526–539. doi: 10.1111/jnc.14463

Figure 8. CRISPR-Cas9 knockdown of GPR75.

Figure 8.

A. Schematic of CRISPR-Cas9 targeting of human GPR75. Two guide RNA’s (gRNA- A, B) were used to create target mutations within the GPR75 gene. B. Analysis of genomic DNA of WT and transfected SH-SY5Y cells. HEK293 cells were used as a comparison. After PCR amplification of the GPR75 coding region (as described in Materials and Methods), the T7E1 assay of mutated cells shows specific cleavage of the band resulting in the expected 555bp + 302bp products for gRNA A and 494bp + 363bp products for gRNA B but not in WT cells.