Table 1.

SH-SY5Y cells express GPR75 but not other CCL5 receptors.

	GPR75		CCR1		CCR3		CCR5
	qPCR	flow cytometry	qPCR	flow cytometry	qPCR	flow cytometry	qPCR	flow cytometry
SH-SY5Y	yes	yes	no	no	no	no	no	no
Human CD4+	yes		yes		yes		yes
THP-1		yes		yes		yes		yes

qPCR and flow cytometry were used to detect expression of the indicated mRNAs and membrane receptors, respectively, in SH-SY5Y cells. CD4 and TH-1 cells were used as positive controls for qPCR and flow cytometry, respectively. Details of the methods are described in Materials and Methods. For qPCR, yes indicates amplification of mRNA of the indicated genes after 40 cycles, no indicates undetectable levels of mRNA for CCR1, CCR3, and CCR5 after 40 cycles. Hypoxanthine phosphoribosyltransferase was used as housekeeping gene. For flow cytometry, no indicates undetectable expression of indicated chemokine receptors on cell membranes.