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. 2018 Jul 10;38(10):1526–1537. doi: 10.1093/treephys/tpy073

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — In-situ Raman mapping of pine tracheids during heartwood formation. (a) Bright field image of a transverse microsection of a fresh pine stem from sapwood to heartwood (17–21st annual ring counted from pith to bark). Raman measurement areas are marked by the numbered rectangles (1–9). (b–d) Raman images (40 × 40 μm) based on bands for (b) cellulose (1076–1110 cm⁻¹), (c) phenolic compounds (1556–1694 cm^–1) and (d) pinosylvins (982–1011 cm^–¹, 1,3,5-substituted aromatic ring). (e) The graph is plotted as an intensity (997 cm^–1) profile along the white dotted lines at d. Legend: CC, cell corner; CML, compound middle lamella; S2 and S3, secondary cell walls; L, Lumen.