Figure 4.

Brevilin A induces intrinsic apoptosis in U87 glioblastoma cells.

Notes: (A) U87 glioblastoma cells were treated with Brevilin A for 24 hours and cellular extracts were subjected to Western blotting analysis for the expression of Bcl-2, Bcl-xl, Bax, and Bak proteins. The expression of cytochrome c was determined in cytosolic extractions as described in Materials and methods. The digital values above the immunoblots show the relative density of the bands normalized to GAPDH from three different experiments. (B) U87 cells were treated with Brevilin A, and the mitochondrial membrane potential was determined using the JC-1 kit. The MMP was calculated by measuring the red/green fluorescence intensity ratio. Columns with different superscript letters differ significantly (P<0.05). (C) The cells were incubated with indicated concentrations of Brevilin A for 24 hours. Following drug treatment, the total cell lysates were prepared and subjected to Western blotting analysis for the expression of cleaved forms of caspase-9, caspase-3, and PARP. GAPDH was used as loading control. The values above the bands show the relative density of the bands normalized to GAPDH from three repeated experiments.