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. 2018 Oct 23;14(10):e1007708. doi: 10.1371/journal.pgen.1007708

Fig 6. gtl1 mutants are compromised in resistance to avirulent PstDC3000 strains and accumulate less SA after PstDC3000_avrRPM1 infection.

Fig 6

A-B) The allelic GTL1 mutants gtl1-2 and gtl1-5 were challenged by the use of PstDC3000_avrRPM1 and PstDC3000_avrRPT2. Results of two GTL1-overexpressing lines (GTL1ox1, GTL1ox2) are shown in S Fig 4E and 4F. Plants, of three biological replicates (n = 30), were spray-inoculated with a bacterial suspension at OD600 0.2, the density of colony-forming units (cfu) was analyzed 2 and 72 hours post inoculation (hpi). Error bars, mean ± SEM, statistical significance was analyzed by Student’s test, asterisks indicate significant differences compared to treated WT, * p≤ 0.05 ** p≤ 0.01, *** p≤ 0.001. C) Quantitative analysis of free salicylic acid by using LC-MS/MS. Box plots are depicted for gtl1-2 and WT, 24 hrs after PstDC3000_avrRPM1 spray-inoculation, untreated conditions are shown in Fig 4H. Boxes showing the interquartile range (IQR) 25st to 75th percentiles, inner square representing the median, whiskers show the SEM, outliers are depicted by dots (Min/Max range). Statistical significance was analyzed by Student’s test. Asterisks indicate significant differences compared to WT,** p≤ 0.01.