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. 2018 Oct 23;13(10):e0206246. doi: 10.1371/journal.pone.0206246

Fig 1. The diagram of ADC construction.

Fig 1

(a) Lysine-based conjugation of mAb-DM1: 1) mAb modification by cross linker Sulfo-SMCC, and 2) conjugation of DM1 with the purified mAb-SMCC. (b) Cysteine-based conjugation of mAb-MMAE using traditional linker: 1) synthesis of Mc-Val-Cit-PABC-PNP linker-MMAE payload, and 2) conjugation of payload with the DTT/TCEP reduced mAb. (c) Cysteine-based conjugation of mAb-MMAE using rebridging linker: 1) synthesis of rebridging linker, 2) synthesis of rebridging linker-MMAE payload, and 3) conjugation of payload with the DTT/TCEP reduced mAb. (d) Structures of free drugs MMAE and DM1.