Figure 3.

Ni‐NTA assay shows greatly reduced GFP(+36) binding to resin after encapsulation within AfFtn. Samples were incubated at 6 μM AfFtn, 12 μM GFP(+36) for 1 h to enable binding of GFP His‐tag to Ni resin. Samples were then centrifuged and the supernatants were analyzed by fluorescence (“load”), followed by three washings of the resin (“wash 1–3”). Increasing [imidazole] to 250 mM dissociated any protein bound to the resin (“elution 1–3”). Green bars are for GFP(+36) alone, navy bars are for AfFtn–GFP(+36). High fluorescence in the load sample for AfFtn–GFP(+36) indicates a lack of binding, due to encapsulation, while high fluorescence in the Elution 1 sample for free GFP(+36) indicates resin binding and release, as expected for a protein with a His‐tag.