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. 2018 Oct 23;27(10):1755–1766. doi: 10.1002/pro.3483

Figure 5.

Figure 5

Non‐superpositively charged GFP cargo is not encapsulated. (A) Native gel electrophoresis of wt‐AfFtn mixed with eGFP at varying stoichiometries. “1” = concentration of 0.6 μM. (B) SEC of wt‐AfFtn–eGFP shows no peak corresponding to encapsulation product, indicating that high charge is needed to induce encapsulation (6 μM AfFtn, 12 μM GFP(+36)). (C) SEC of wt‐AfFtn–GFP(−30) shows no peak corresponding to encapsulation product, indicating that charge complementarity between the AfFtn cage interior and the cargo protein is important for encapsulation (6 μM AfFtn, 12 μM GFP(+36)).