Table 2.
Thermodynamic analysis of the interaction between the different TEAD4 variants and wt/Ser94AlaYAP. The different parameters were measured by Isothermal Titration Calorimetry at 295°K. Average values and standard errors are indicated (n ≥ 2)
| wtYAP | Ser94AlaYAP | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TEAD4 | n a | K d (nM) | ΔG (kcal/mol) | ΔH obs (kcal/mol) | −TΔS
(kcal/mol) |
n | K d (nM) | ΔG (kcal/mol) | ΔH obs (kcal/mol) | −TΔS (kcal/mol) |
| wt | 1.05 ± 0.02 | 37 ± 5 | −10.15 ± 0.05 | −13.0 ± 0.1 | 2.8 ± 0.1 | 1.1 ± 0.00 | 3300 ± 400 | −7.5 ± 0.1 | −11.0 ± 0.1 | 3.52 ± 0.01 |
| Tyr429Phe | 0.96 ± 0.01 | 92 ± 3 | −9.61 ± 0.02 | −13.3 ± 0.3 | 3.7 ± 0.4 | 0.93 ± 0.02 | 210 ± 20 | −9.1 ± 0.1 | −11.10 ± 0.04 | 2.0 ± 0.1 |
| Glu263Ala | 0.99 ± 0.03 | 212 ± 2 | −9.11 ± 0.00 | −13.2 ± 0.2 | 4.1 ± 0.2 | 0.98 ± 0.03 | 4160 ± 300 | −7.35 ± 0.04 | −11.4 ± 0.1 | 4.1 ± 0.2 |
| Glu263Ala‐Tyr429Phe | 1.06 ± 0.04 | 289 ± 11 | −8.93 ± 0.02 | −10.85 ± 0.05 | 1.93 ± 0.01 | 1.05 ± 0.03 | 291 ± 40 | −8.9 ± 0.1 | −10.60 ± 0.00 | 1.7 ± 0.1 |
a
n: stoichiometry.