Table 1.
Sequencing results of PCR amplicons from four genetic regions of Halarachne halichoeri from southern sea otters, Enhydra lutris nereis, from California.
| Genes | Primers | Sequences (5‘-3‘) | Source | Amplicon size (bp) | BLAST Results (percent homology) |
|---|---|---|---|---|---|
| 18S | F: 31F1 R: 344R2 |
CGCGAATGGCTCATTAAATC GCCTTCCTTGGATGTGGTAG |
Foley et al. (2013) | 289 |
Dinogamasus 98%, Ichoronyssus 98%, Ornithonyssus 98% |
| 16S I | F: 16S + 1 R: 16S-2 |
CTGCTCAATGATTTTTTAAATTGCTGTGG TTACGCTGTTATCCCTAGAG |
Black and Piesman (1994) | 266–267 | Stylochyrus 78% |
| 16S II | F: 16S + 2 R: 16S-1 |
TTGGGCAAGAAGACCCTATGAA CCGGTCTGAACTCAGATCAAGT |
Black and Piesman (1994) | 245–246 |
Ornithonyssus 85%, Dermanyssus 84% |
| 28S | F: 43F R: 929R |
GCTGCGAGTGAACTGGAATCAAGCCT AGGTCACCATCTTTCGGGTC |
Dowling and Oconnor (2010) | 440–587 | Gaeolaelaps 92%, Cosmolaelaps 91% |
| ITS1-2 | F: ITS-1F R: ITS-1R I: ITS-1int |
AGAGGAAGTAAAAGTCGTAACAAG ATATGCTTAAATTCAGGGGG GGTCTTCACATYTGATTTCAG |
Morelli and Spicer (2007) | 469–475 | Coleolaelaps 89%, Tropilaelaps 89% |