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. 2018 Oct 23;8:15632. doi: 10.1038/s41598-018-33977-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Single-particle analysis of r-EhV-ATPase by ZPP cryo-EM. (a) Representative 2D class averages of r-EhV-ATPase particles. 2D class averages were calculated from ~120,000 particles. Scale bar: 10 nm. (b) Gold-standard Fourier shell correlation (GS-FSC) curve for the final 3D reconstruction of r-EhV-ATPase. Resolution was estimated as 17.3 Å using GS-FSC criteria. (c) Angular distribution of individual images in the final 3D reconstruction, showing unbiased image sampling was achieved.