Fig. 7.

eRIC identifies m6A-responsive RBPs in vivo. a m6A dot blot of Jurkat cells treated with 0.5 mM DMOG or vehicle for 6 h, using two independent antibodies (Antibody 1: Abcam, Antibody 2: SySy). Serial dilutions of poly(A) RNA were blotted as indicated on the right. A quantification of the signal intensity based on image analysis is shown below each dot. Intensity is expressed relative to the lowest signal in the control. A representative blot of three biologically independent experiments is shown. b Overlap between DMOG-responsive RBPs identified by eRIC/RIC and m6A-regulated RBPs previously reported by Edupuganti et al.²¹ or Arguello et al.²⁰. Between brackets are the number of proteins with directions of DMOG-induced changes that coincide with previous reports. c Normalized signal sum in eRIC and RIC samples of representative examples of reported m6A readers (left), m6A-repelled RBPs (middle), and RBPs insensitive to m6A (right). −UV non-irradiated controls, +UV irradiated samples. eRIC and RIC values are expressed relative to the respective untreated control (−UV, DMSO). Data are shown as mean from two biologically independent experiments. Asterisk (*) indicates FDR < 0.05 (moderated t test)