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. 2018 Oct 23;8:15650. doi: 10.1038/s41598-018-34040-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Our dual-view on-chip holographic imaging platform consists of a CMOS image sensor connected to a frame grabber operating at ~300 fps and two fiber-coupled LEDs emitting green light (~525 nm center-wavelength), placed in mirror-symmetry with a ~18° tilt³³. (b) The sample chamber placed above the image sensor with a periodic light-blocking structure as the top layer³³. (c) The periodic light-blocking structure spatially separates the pair of holographic projections across the image sensor, fully utilizing the dynamic range of the CMOS imager, achieving better sensitivity to image weakly-scattering flagellar structures of sperm cells³³.