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. 2018 Oct 23;8:15654. doi: 10.1038/s41598-018-34047-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Acute inhibition of A6 neurons and effect on the baseline ventilatory, inspiratory and expiratory parameters of conscious adult rats. (A) Raw and integrated (∫) records of Dia_EMG and Abd_EMG activities and barometric respiratory movements, as well as fR, V_T and V_Edata from one rat, in which the LC was transduced with PRSx8-AlstR-GFP-LVV before and after Alst application into the lateral ventricle. Note the absence of changes in baseline inspiratory and expiratory activities, in the ventilatory parameters, as well as in the number of sighs (red lines) after acute inhibition of A6 neurons. Summary of data showing the changes in the fR (B), V_T (C), VE (D) and in the number of sighs (E) after application of Alst.