Fig. 3.

TDP-43 and Lilli colocalize at the 93D locus on polytene chromosomes. a Immunostainings of DAPI (white), TDP-43-YFP (green), and Lilli (magenta) show partial overlap of TDP-43-YFP and Lilli on chromosomes from salivary glands expressing TDP-43-YFP. Sixteen sites of consistent colocalization are indicated (bottom, see also Supplementary Table 1). b Upon heat shock, Lilli localizes to major heat shock loci and colocalizes with TDP-43-YFP at the 93D locus (enlarged at the upper right corner). See also Supplementary Table 2. For a, b, scale bars: 10 μm. Genotype: sgs3-GAL4/UAS-TDP-43-YFP. c Venn diagram illustrates the numbers of genes bound by TBPH and Lilli. d A ChIP-seq profile of TBPH (top, blue) and Lilli (bottom, orange) shows overlap at the Hsrω transcription start site. Bar intervals indicate called peaks in TBPH (light blue, top) and Lilli (light orange, bottom). The TBPH profile is one of four biological replicates that show called peaks at the Hsrω transcription start site