Figure 4.

Gap19 suppressed the TLR4 signaling pathway and inflammatory cytokines expression in vivo. (A) Brain tissues were collected after 24 h reperfusion, and TLR4, MyD88 and NF-κB p-p65 levels were determined by Western blot. Gap19 treatment inhibited the increment of TLR4 (B), MyD88 (C) and NF-κB p-p65 (E) expression. (D) Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) protein expression was also lower in Gap19-treated mice than I/R mice 24 h post-reperfusion (F,G). Data represent the mean ± SEM (n = 3 mice/group). *P < 0.05 vs. I/R group.