FIGURE 2.

Intrinsic apoptosis pathway by CDDP is mediated by Mcl-1 in MKN45 cells. (A) Bcl-2 protein family members were detected by western blot in AGS and MKN45 cells treated with CDDP (10 μg/ml) at the indicated periods of time. Mcl-1, Bid, and Bad were detected by using specific antibodies. (B) Cells were treated as in panel (A). ERK, JNK, p38 (phosphorylated and total forms), and DUSP1 expression were detected by using specific antibodies. (C) AGS and MKN45 cells were pretreated during 30 min with 10 μM JNK Inhibitor II or p38 inhibitor (SB203580) before CDDP treatment. Mcl-1 was detected by western blot at the indicated times after treatment. α-Tubulin was used as loading control. WB images are representative of the results obtained in three different experiments made in the same conditions.