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. 2018 Oct 17;9:616. doi: 10.3389/fendo.2018.00616

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Copyright © 2018 Musutova, Elkalaf, Klubickova, Koc, Povysil, Rambousek, Volckaert, Duska, Trinh, Kalous, Trnka, Balusikova, Kovar and Polak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipid co-localization imaging and total intracellular lipid content. (A) A representative example of a microscope image of lipids stained with BODIPY 493/503 (green) forming distinct vesicular structures marked by arrows (A), staining (red) for peroxisome-specific protein PMP70 (B), merged image of lipid and PMP70 staining (C) and merged image of lipid, PMP70, and nuclei staining (blue) with Hoechst 33258 (D). (B) Total lipid content during hypoxia and after metformin treatment The effect of hypoxic exposure (4% O₂) and 7-day metformin administration on total lipid content in differentiated L6 myotubes. ^*p < 0.05 for comparison with vehicle-treated cells, ^#p < 0.05 for comparison with control exposures (20% O₂), n = 6. Two-way ANOVA was used to explore interaction between exposure to hypoxia and metformin treatment.