FIGURE 4.

Gremlin via VEGFR2 regulates cells migration and proliferation in wound healing experiments. A monolayer of epithelial cells (HK2 cell line) was used as starting point. A single scrape wound was made in all cells and then they were stimulated with Gremlin (10 ng/ml) in the presence or absence of VEGFR2 kinase inhibitor SU5416 (5 μM, preincubated 1 h before). After 72 h, cells were evaluated under inverted microscopy. (A) Representative images of wound healing experiments and (B) their quantification. Results are expressed as mean ± SEM of four independent experiments, done by duplicate. ^∗p < 0.05 vs. control; ^#p < 0.05 vs. Gremlin.