FIGURE 8.

Effect of Notch inhibition on Gremlin-induced changes in wound healing assays. A monolayer of epithelial cells (HK2 cell line) was used as starting point. A single scrape wound was made. Then cells were preincubated with DAPT (30 nM) or vehicle before stimulation with Gremlin (10 ng/ml). After 72 h, cells were evaluated under an inverted microscopy. (A) Representative images of wound healing experiments and (B) their quantification. Results are expressed as mean ± SEM of four independent experiments, performed by duplicate. ^∗p < 0.05 vs. control; ^#p < 0.05 vs. Gremlin.