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. 2018 Oct 17;9:2398. doi: 10.3389/fimmu.2018.02398

Figure 3.

Figure 3

Dopamine inhibits cytokine production in iNKT cells through D1-like receptors. (A) mRNA level and fragments of various dopamine receptors in iNKT cells. (B) Influences of indicated selective receptor agonists (20 μM) on production of IL4 and IFNγ in iNKT cells. iNKT cells were activated by α-GalCer-pulsed RBL.CD1d cells. (C) Influences of SCH23390, antagonist of D1-like receptors, on IL4 and IFNγ production in iNKT cells. (D) Influences of A68930, agonist of D1-like receptors, on IL4 and IFNγ production in iNKT cells pretreated with or without SCH23390 for 3 h. (E) Protein levels of DRD1, DRD5, and β-actin in iNKT cells. Brain was used as positive control. Data are representative of more than three independent experiments. (F) Intracellular staining of IL4 and IFNγ in hepatic and splenic iNKT cells after injecting α-GalCer (2 μg/mouse) with or without A68930 (8 mg/kg) for 4 h. In negative controls, mice were injected with PBS buffer (n = 8 mice per group). (G) Percentages of IL4+ and IFNγ+ iNKT cells as in (F). Error bars represent SEM. *P < 0.05, **P < 0.01, ***P < 0.001. DR, dopamine receptor.