Table 2.
Reaction conditions tested for teicoplanin conjugation to NP-APTES via EDC/NHS chemistry in 30 mM MES, pH 6.0.
| Teicoplanin (μg/mL) | EDC (mM) | NHS (mM) | Temperature (°C) | Time (h) | Yield (%) |
|---|---|---|---|---|---|
| 100 | 26 | 13 | 4 | 2 | 10 ± 0.7 |
| 100 | 26 | 13 | 4 | 4 | 10 ± 1.5 |
| 100 | 26 | 13 | 4 | 6 | 12 ± 1.0 |
| 100 | 26 | 13 | 25 | 2 | 25 ± 0.5 |
| 100 | 26 | 13 | 25 | 4 | 30 ± 0.4 |
| 100 | 26 | 13 | 25 | 6 | 65 ± 0.8 |
| 100 | 13 | 26 | 25 | 2 | 70 ± 1.7 |
| 100 | 13 | 26 | 25 | 4 | 85 ± 1.4 |
| 50 | 13 | 26 | 25 | 6 | 100 ± 1.2 |
| 100 | 13 | 26 | 25 | 6 | 100 ± 0.7 |
| 500 | 13 | 26 | 25 | 6 | 90 ± 0.9 |
| 1000 | 13 | 26 | 25 | 6 | 50 ± 0.5 |
NP-APTES were used at a concentration of 4 mg/mL. Conjugation yield was calculated by estimating the amount of residual teicoplanin in the reaction medium by HPLC.