Table 1.
Effects of CACHD1 and α2δ-1 on biophysical properties of CaV3 subtypes
| Gmax (pS/pF) | V1/2 (mV) | k (mV) | τ Activation (ms)a | τ Inactivation (ms)b | |
|---|---|---|---|---|---|
| CaV3.1 (18) | 628 ± 70 | −34.5 ± 0.8 (30) | 5.4 ± 0.1 (30) | 2.0 ± 0.1 | 25.8 ± 2.0 |
| CaV3.1/CACHD1 (19) | 944 ± 90* | −36.3 ± 0.9 (29) | 5.6 ± 0.2 (29) | 2.0 ± 0.2 | 22.2 ± 4.8 |
| CaV3.1/α2δ-1 (13) | 672 ± 90 | −35.7 ± 1.4 | 5.6 ± 0.3 | 3.3 ± 0.2** | 18.9 ± 0.86*** |
| CaV3.2 (13) | 596 ± 120 | −34.4 ± 2.4 | 5.7 ± 0.2 | 7.1 ± 0.40 | 33.3 ± 0.97 |
| CaV3.2/CACHD1 (15) | 1060 ± 140**** | −33.4 ± 0.8 | 5.9 ± 0.2 | 5.9 ± 0.38 | 32.0 ± 1.6 |
| CaV3.3 (12) | 573 ± 88 | −36.1 ± 1.2 | 4.3 ± 0.2 | 24.4 ± 1.9 | 134 ± 12 |
| CaV3.3/CACHD1 (10) | 849 ± 78***** | −38.9 ± 1.6 | 4.0 ± 0.3 | 28.5 ± 3.4 | 126 ± 8.3 |
In all cases, comparisons were performed in culture-matched experiments. Numbers in parenthesis represents the number of cells each from a minimum of five separate transfections.
aτ activation was measured at −25 mV in all cases.
bτ inactivation was measured at −20 mV for CaV3.1 and CaV3.2, and at −30 mV for CaV3.3.
*p < 0.05 vs CaV3.1 (one-way ANOVA with Bonferroni post hoc test);
**p < 0.05,
***p < 0.05, vs CaV3.1 (two-way ANOVA with Bonferroni post hoc test);
****p < 0.05 vs CaV3.2 (two-tailed unpaired Student's t test);
*****p < 0.05 vs CaV3.3 (two-tailed unpaired Student's t test).