Figure 4. Effect of amentoflavone on tumor progression-associated proteins modulated by extracellular signal-regulated kinase (ERK)/nuclear factorĸB (NF-ĸB) in SK-Hep1/luc2 tumor-bearing mice. Protein levels of phosphorylated (P)ERK, NF-ĸB p65 (Ser536), matrix metallopeptidase 9 (MMP- 9), vascular endothelial growth factor (VEGF), cyclin-D1, myeloid leukemia cell differentiation protein (MCL-1), cellular FADD-like IL-1β-converting enzyme-inhibitory protein (C-FLIP), and X-linked inhibitor of apoptosis protein (XIAP) in tumor tissues were investigated by immunohistochemistry. A: Image at magnification of 100×. B: Quantification of protein level. C: Pathological examination of liver tissues obtained from mice sacrificed on day 14 after treatment was evaluated by hematoxylin-eosin staining. aSignificantly different at p<0.01 compared to the vehicle-treated group.