Figure 2. Microbubble charactarization. A: Representative bright-light image of microbubbles. B: Representative fluorescence image of themicrobubbles conjugated with rhodamine-labeled Endostar. C: Representative fluorescence image of microbubbles conjugated with fluoresceinisothiocyanate-labeled antibody to intercellular cell adhesion molecule 1 (ICAM-1). Magnification: 400×. Flow cytometric analysis of: D:Unconjugated microbubbles (MB), E: ICAM-1-targeted microbubbles (MBi) and F: ICAM-1-targeted and endostar-loaded therapeutic microbubbles(MBie). G: Conjugation rate of MBi and MBie .
