Fig. 4.

Different responses from the repressive versus non-repressive TAD genes upon knockdown or mutation of MSL complex components. Boxplots represent gene expression changes in log2 scale from depletion of MSL components in Drosophila S2 cells (a–l) or roX mutation in male larvae (m–n). Plots are based on four independent studies [23, 47, 54, 88], which used either microarray (a–h and m–n) or RNA-Seq technology (i–l). a, b Differential gene expression from mof knockdown cells. Changes from the repressive TADs (left three columns, LAD, Null and Black) as well as MOF binding, or Histone H4K16 acetylation regions are presented. c–h Results from msl-1, msl-2, or msl-3 knockdown. i, j Results from mof knockdown, measured by RNA-Seq analysis. k, l msl-2 knockdown. m, n Results from roX1 and roX2 null mutant larvae, measured by microarrays. a, c, e, g, i, k, m Changes from X chromosome genes. b, d, f, h, j, l, n Changes from autosomal genes. *p < 0.05, **p < 0.01, ***p < 0.001