Table 3.
Taxonomic classification of BEI
| Centroids full-length | Centroids v3-v5 | Clustered from V3-V5 truncated reads (Top 19) | Mothur/Silva full-length (Top 19) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Species | Genus conf | Species conf | Species | Genus conf | Species conf | Species | Genus conf | Species conf | Genus | Genus conf |
| Acinetobacter_baumannii | 0.98 | 0.94 | Acinetobacter_baumannii | 0.88 | 0.42 | Acinetobacter_baumannii | 0.8784 | 0.42 | Acinetobacter | 100 |
| Actinomyces_odontolyticus | 0.99 | 0.94 | Actinomyces_odontolyticus | 0.97 | 0.62 | Actinomyces_odontolyticus | 0.9728 | 0.62 | Actinomyces | 100 |
| Bacillus_anthracis | 0.99 | 0.49 | Bacillus_cereus | 0.96 | 0.42 | Bacillus_cereus | 0.963 | 0.31 | Bacillus | 100 |
| Bacteroides_vulgatus | 0.99 | 0.94 | Bacteroides_vulgatus | 0.98 | 0.90 | Bacteroides_vulgatus | 0.9847 | 0.90 | Bacteroides | 100 |
| Clostridium_beijerinckii | 0.99 | 0.72 | Clostridium_roseum | 0.97 | 0.42 | Clostridium_roseum | 0.965 | 0.31 | Clostridium_sensu_stricto | 100 |
| Deinococcus_radiodurans | 0.99 | 0.94 | Deinococcus_radiodurans | 1.00 | 0.90 | Deinococcus_radiodurans | 0.9994 | 0.90 | Deinococcus | 100 |
| Enterococcus_faecalis | 0.99 | 0.94 | Enterococcus_faecalis | 0.88 | 0.83 | Enterococcus_faecalis | 0.8784 | 0.83 | Enterococcus | 100 |
| Shigella_flexneri | 0.69 | 0.49 | Shigella_flexneri | 0.67 | 0.42 | Shigella_flexneri | 0.6072 | 0.31 | Escherichia_Shigella | 100 |
| Helicobacter_pylori | 0.99 | 0.93 | Helicobacter_pylori | 0.98 | 0.90 | Helicobacter_pylori | 0.9787 | 0.90 | Helicobacter | 100 |
| Lactobacillus_gasseri | 0.99 | 0.72 | Lactobacillus_gasseri | 0.97 | 0.42 | Lactobacillus_gasseri | 0.9728 | 0.42 | Lactobacillus | 100 |
| Listeria_monocytogenes | 0.99 | 0.72 | Listeria_ivanovii | 0.96 | 0.42 | Listeria_ivanovii | 0.961 | 0.31 | Listeria | 100 |
| Neisseria_meningitidis | 0.97 | 0.93 | Neisseria_meningitidis | 0.95 | 0.62 | Neisseria_meningitidis | 0.9533 | 0.62 | Neisseria | 100 |
| Propionibacterium_acnes | 0.97 | 0.96 | Propionibacterium_acnes | 0.97 | 0.91 | Propionibacterium_acnes | 0.965 | 0.91 | Propionibacterium | 100 |
| Pseudomonas_aeruginosa | 0.97 | 0.93 | Pseudomonas_aeruginosa | 0.93 | 0.62 | Pseudomonas_aeruginosa | 0.9329 | 0.62 | Pseudomonas | 100 |
| Rhodobacter_sphaeroides | 0.97 | 0.60 | Rhodobacter_sphaeroides | 0.92 | 0.42 | Rhodobacter_sphaeroides | 0.9228 | 0.31 | Rhodobacter | 100 |
| Staphylococcus_epidermidis | 0.99 | 0.72 | Staphylococcus_epidermidis | 0.67 | 0.42 | Staphylococcus_epidermidis | 0.6661 | 0.31 | Staphylococcus | 100 |
| Streptococcus_agalactiae | 0.99 | 0.95 | Streptococcus_agalactiae | 0.97 | 0.90 | Streptococcus_agalactiae | 0.9728 | 0.90 | Streptococcus | 100 |
| Streptococcus_mutans | 0.99 | 0.95 | Streptococcus_mutans | 0.98 | 0.90 | Streptococcus_mutans | 0.9768 | 0.90 | Streptococcus | 100 |
| Streptococcus_pneumoniae | 0.99 | 0.49 | Streptococcus_pneumoniae | 0.98 | 0.42 | Streptococcus_pneumoniae | 0.9768 | 0.42 | Streptococcus | 100 |
Classification results from the BEI mock community. The performance of MCSMRT FL16S centroid OTU assignments (column 1), centroids truncated to V3-V5 (column 2), top 19 centroids found when clustering V3-V5 truncation reads (column 3), and the top 19 OTUs identified using Mothur/Silva with FL16S reads (column 4)