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. 2018 Oct 24;4:39. doi: 10.1186/s40942-018-0143-x

Table 1.

Anti-VEGF antibody recovered mass in aflibercept and ziv-aflibercept subjected to different storage conditions

Anti-VEGF recovered mass (mg)
Aflibercept Ziv-aflibercept
Expected mass 2.05 1.28
Pre-assay 2.03 ± 0.02 1.33 ± 0.01
14 days/4 °C 2.08 ± 0.10 1.17 ± 0.14
14 days/− 8 °C 2.21 ± 0.02 1.26 ± 0.11
28 days/4 °C 2.09 ± 0.17 1.28 ± 0.00
28 days/− 8 °C 1.97 ± 0.10 1.25 ± 0.06

Different samples of aflibercept and ziv-aflibercept were added to ELISA plates and detected by HRP-conjugated anti-human IgG and H2O2-OPD substrate. Anti-VEGF quantification (mg) was based on a standard curve performed with both drugs. The drugs were initially diluted in saline (1:256,000 for aflibercept; 1:128,000 for ziv-aflibercept). Statistical analysis was performed comparing the samples to the moment condition of each drug. Representative result of three experiments (mean)

The data are expressed as the mean ± SD