Table 1.
Anti-VEGF recovered mass (mg) | ||
---|---|---|
Aflibercept | Ziv-aflibercept | |
Expected mass | 2.05 | 1.28 |
Pre-assay | 2.03 ± 0.02 | 1.33 ± 0.01 |
14 days/4 °C | 2.08 ± 0.10 | 1.17 ± 0.14 |
14 days/− 8 °C | 2.21 ± 0.02 | 1.26 ± 0.11 |
28 days/4 °C | 2.09 ± 0.17 | 1.28 ± 0.00 |
28 days/− 8 °C | 1.97 ± 0.10 | 1.25 ± 0.06 |
Different samples of aflibercept and ziv-aflibercept were added to ELISA plates and detected by HRP-conjugated anti-human IgG and H2O2-OPD substrate. Anti-VEGF quantification (mg) was based on a standard curve performed with both drugs. The drugs were initially diluted in saline (1:256,000 for aflibercept; 1:128,000 for ziv-aflibercept). Statistical analysis was performed comparing the samples to the moment condition of each drug. Representative result of three experiments (mean)
The data are expressed as the mean ± SD