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. 2018 Oct 23;11:554. doi: 10.1186/s13071-018-3134-8

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Coding sequence (mRNA sequence) of Aphanomyces invadans’ hypothetical protein (GenBank: XM_008877667) contains 1388 nucleotides. The designed gRNA2 site is highlighted in green and the PAM site in grey. Forward and reverse primer 2 (product size 214 bp) sites are highlighted in yellow. The point mutation (deletion) located at the 5th nt from the PAM site is shown in red within the gRNA2 sequence