Table 1.
PCR primers designed for each guide RNA. Three PCR primer sets corresponding to each gRNA were designed based on the A. invadans target gene (GenBank: XM_008877667) to detect the genomic DNA mutation, caused by CRISPR/Cas9
| Name | Position | Expected product size (bp) | Sequence (5'- 3') | |
|---|---|---|---|---|
| Primer set 1 | AiCr1F | 35–54 | 211 | GACTCCGACCTTGACGATGC |
| AiCr1R | 227–246 | AAAGAGGAATGAGGCGGAGG | ||
| Primer set 2 | AiCr2F | 884–904 | 214 | CCCACACCATGGGAACGATTG |
| AiCr2R | 1080–1098 | TTGAGACGAGCCCCACGAG | ||
| Primer set 3 | AiCr3R | 1572–1591 | 209 | GTTCGGCCGTATTAACGCCA |
| AiCr3F | 1763–1781 | CTTGGTACATGGCTGCCCA |