Fig. 2.

Multiple alignment of the JrNF-Y family was performed by Clustal X. Conserved regions of all JrNF-Y proteins and Mus musculus NF-Y proteins (NF-YMouse) are shown. NF-YAMouse, NF-YBMouse, NF-YCMouse were respectively compared with the JrNF-YA, JrNF-YB, JrNF-YC subfamilies. Regions required for DNA binding or interacted with NF-YA, NF-YB and NF-YC subfamilies were previously defined in mammals and yeast. Amino acids in black boxes/white letters were identical in 100% of all aligned sequences. Amino acid sequences with the symbol “#” were conserved in walnut but divided when compared with mouse. Amino acid sequences with the symbol “x” were unique in mouse, and only in the NF-YC subfamily