Table 1.
Composition of culture media for domesticated einkorn (T.monococcum L.) tissue culturea
| Media (procedure) | Phytohormones, mg/l | Carbohydrates, % | PPT, mg/l | Duration period | ||||
|---|---|---|---|---|---|---|---|---|
| Dicamba | Daminozide | TDZ | GA3 | Sucrose | Mannitol | |||
| Callus induction medium basal | 3 | 50 | 0.25 | 3 | 5–15 days | |||
| Callus induction medium modified | 3 | 50 | 0.1 | 3 | ||||
| Osmoticum medium | 3 | 50 | 3 | 7.3 | 20 hours | |||
| Callus selection medium A | 3 | 25 | 3 | 3 | 6 weeks | |||
| Callus selection medium B | 3 | 3 | 3 | |||||
| Callus proliferation medium | 1.5 | 3 | 3 | 3 weeks | ||||
| Differentiation medium | 0.5 | 3 | 3 | 2–3 weeks | ||||
| Regeneration medium | 3 | 3 | 3–5 weeks | |||||
| Rooting medium | 2 | 2 | 2–4 weeks | |||||
| Embryo germination medium | 0.1 | 2 | 5–10 days | |||||
aMS medium consisted of MS basal salts and vitamins supplemented with 150 mg/l L-asparagine and 7 g/l agar was used as basic medium in all cases