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Gene expression analysis of matrix metalloproteinase 1 (MMP1) and α1(I)-procollagen (COL1A1) assessed by qPCR. The histograms show the modulation of the MMP1 (a) and COL1A1 (b) mRNAs by oxygen tension (21% O2 vs 5% O2) at matched cell passages in young (Y) and old (O) HDF. The genes were normalized vs GAPDH/β-actin/SDHA and the results are reported as normalized fold expression. Error bars represent SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
